vector plpcx Search Results


plpcx retroviral vector  (Becton Dickinson)
90
Becton Dickinson plpcx retroviral vector
Plpcx Retroviral Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plpcx retroviral vector/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
plpcx retroviral vector - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
plpcx vector  (Becton Dickinson)
90
Becton Dickinson plpcx vector
Establishment of hTERT -immortalized Sca-1+ CSC lines. ( A ) Schematic structure of <t>pLPCX-</t> hTERT -IRES- GFP ( top ). Correct construction of pLPCX- hTERT -IRES- GFP was confirmed by digestion with restriction enzymes ( bottom <t>),</t> <t>Bgl</t> II (Lane 2), Cla I (Lane 3), or Bgl II /Cla I (Lane 4). Lane M: λ/ Hind III marker. Lane 1: Supercoiled pLPCX- hTER T-IRES- GFP ; ( B ) retroviruses were produced in 293GPG packaging cells by transfection with a retroviral vector encoding hTERT -IRES- GFP using Lipofectamine 2000. At 48 h post transfection, expression of GFP (green), a reporter gene was monitored in 293GPG cells. Scale bars = 20 μm; ( C ) Sca-1+ CSCs transduced with retroviruses expressing hTERT -IRES- GFP (green) were selected in 96-well plates at a single cell level by limiting dilution for 12 days; and ( D ) two putative Sca-1+ CSC lines were finally selected. Nuclei were stained with DAPI (blue).
Plpcx Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plpcx vector/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
plpcx vector - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Establishment of hTERT -immortalized Sca-1+ CSC lines. ( A ) Schematic structure of pLPCX- hTERT -IRES- GFP ( top ). Correct construction of pLPCX- hTERT -IRES- GFP was confirmed by digestion with restriction enzymes ( bottom ), Bgl II (Lane 2), Cla I (Lane 3), or Bgl II /Cla I (Lane 4). Lane M: λ/ Hind III marker. Lane 1: Supercoiled pLPCX- hTER T-IRES- GFP ; ( B ) retroviruses were produced in 293GPG packaging cells by transfection with a retroviral vector encoding hTERT -IRES- GFP using Lipofectamine 2000. At 48 h post transfection, expression of GFP (green), a reporter gene was monitored in 293GPG cells. Scale bars = 20 μm; ( C ) Sca-1+ CSCs transduced with retroviruses expressing hTERT -IRES- GFP (green) were selected in 96-well plates at a single cell level by limiting dilution for 12 days; and ( D ) two putative Sca-1+ CSC lines were finally selected. Nuclei were stained with DAPI (blue).

Journal: International Journal of Molecular Sciences

Article Title: Cardiac Stem Cell Secretome Protects Cardiomyocytes from Hypoxic Injury Partly via Monocyte Chemotactic Protein-1-Dependent Mechanism

doi: 10.3390/ijms17060800

Figure Lengend Snippet: Establishment of hTERT -immortalized Sca-1+ CSC lines. ( A ) Schematic structure of pLPCX- hTERT -IRES- GFP ( top ). Correct construction of pLPCX- hTERT -IRES- GFP was confirmed by digestion with restriction enzymes ( bottom ), Bgl II (Lane 2), Cla I (Lane 3), or Bgl II /Cla I (Lane 4). Lane M: λ/ Hind III marker. Lane 1: Supercoiled pLPCX- hTER T-IRES- GFP ; ( B ) retroviruses were produced in 293GPG packaging cells by transfection with a retroviral vector encoding hTERT -IRES- GFP using Lipofectamine 2000. At 48 h post transfection, expression of GFP (green), a reporter gene was monitored in 293GPG cells. Scale bars = 20 μm; ( C ) Sca-1+ CSCs transduced with retroviruses expressing hTERT -IRES- GFP (green) were selected in 96-well plates at a single cell level by limiting dilution for 12 days; and ( D ) two putative Sca-1+ CSC lines were finally selected. Nuclei were stained with DAPI (blue).

Article Snippet: Finally, the Bgl II- Cla I digested PCR fragment containing hTERT -IRES- GFP cDNA was finally inserted into the Bgl II- Cla I site of the pLPCX vector (BD Biosciences).

Techniques: Marker, Produced, Transfection, Plasmid Preparation, Expressing, Transduction, Staining